高分辨率活細胞生物成像的最佳選擇

LiT LBS激光片層掃描顯微鏡將幫助生物學家以前所未有的靈敏度，分辨率以及成像速度解讀活體樣品的三維動態過程。 Litone I．BS系統使用了最前沿的光學和工程技術來產生一束超薄的線性貝塞爾片層光（ Line Bessel Sheet，簡稱LBS），并用它來實現對生物樣品的高精度光學層析。此項專利技術的應用不僅顯著提高了片層光顯微系統的成像分辦率，而且允許系統使用超弱的激發光便可從樣品中獲得足夠的信號強度，所以極大的減弱了樣品在成像時承受的光毒性，延長了樣品的有效觀測時間，以此幫助觀測者獲得更多高質量的成像數據。

LIT LBS激光片層掃描顯微系統
開創了活細胞五維生物成像的時代
3維空間＋1維時間＋1維顏色

超越激光共聚焦顯微技術

超越傳統激光片層掃描顯微技術

光毒性減弱1000倍

動態拍攝速度提高1000倍

軸向分辨率提高2倍

高速活細胞成像

拍攝速度可達500幅每秒
 

高分辨率三維結構成像
250nm橫向分辨率
350nm軸向分辨率

部分參考文獻： 

• 2018

• Sze Cheung Lau, Hoi Chun Chiu, Luwei Zhao, Teng Zhao, M. M. T. Loy, and Shengwang Du, “An integrated single- and two-photon non-diffracting light-sheet microscope,” Rev. Sci. Instrum. 89, 043701(2018); arXiv:1712.02521 [physics.ins-det].

•  Aifang Cheng, Teng Zhao, Kai-Hei Tse, Hei-Man Chow, Yong Cui, Liwen Jiang, Shengwang Du, Michael M. T. Loy, and Karl Herrup, “ATM and ATR play complementary roles in the behavior of excitatory and inhibitory vesicle populations,” Proc. Natl. Acad. Sci. USA (PNAS) 115, E292 (2018).
  [HKUST Press Release: “HKUST Scientists Reveal How Human Brains Keep Balance“]2017

• 2017

• Franco K. C. Au, Yue Jia, Kai Jiang, Ilya Grigoriev, Bill K. T. Hau, Yuehong Shen, Shengwang Du, Anna Akhmanova, and Robert Z. Qi, “GAS2L1 is a centriole-associated protein required for centrosome dynamics and disjunction,” Developmental Cell 40, 81 (2017)

• Huiqin Huang, Xiaochen Lin, Zhuoyi Liang, Teng Zhao, Shengwang Du, Michael M. T. Loy, Kwok-On Lai, Amy K. Y. Fu, and Nancy Y. Ip, “Cdk5-dependent phosphorylation of liprinα1 mediates neuronal activity-dependent synapse development,” Proc. Natl. Acad. Sci. USA (PNAS) 114, E6992 (2017)

• 2016

•  Hao Wang* Visualizing Plant Cells in A Brand New Way. Molecular Plant 9, 633-635(2016).

• Graham Vail, Aifang Cheng, Yu Ray Han, Teng Zhao, Shengwang Du, Michael M.T. Loy, Karl Herrup, and Mark R. Plummer, “ATM protein is located on presynaptic vesicles and its deficit leads to failures in synaptic plasticity,” J. Neurophysiol. 116, 201 (2016) 

• Hao Wang, Xiaohong Zhuang, Xiangfeng Wang, Angus Ho Yin Law, Teng Zhao, Shengwang Du, Michael M. T. Loy, and Liwen Jiang, “A distinct pathway for polar exocytosis in plant Cell wall formation1“, Plant Physiology 172, 1003 (2016) 

• Teng Zhao, Sze Cheung Lau, Ying Wang, Yumian Su, Hao Wang, Aifang Cheng, Karl Herrup, Nancy Y. Ip, Shengwang Du, and M. M. T. Loy, “Multicolor 4D fluorescence microscopy using ultrathin Bessel light sheets,” Sci. Rep. 6, 26159 (2016).